Figure 7.

L- and D-serine rescue defects in de novo fatty acid synthesis and differentiation

(A) Expression of GFAP (red) in differentiating NSCs from WT and SR^−/− mice.

(B) Expression of GFAP in differentiating NSCs from WT and SR^−/− mice cultured in the presence of equimolar mixture of L-serine and D-serine (100 μM). Scale bar, 20 μm.

(C) Expression of PSPH in NSCs treated with L-serine and D-serine.

(D) Levels of malonyl-CoA (ng/mL) in NSCs cultured from SR^flox/flox and nestin-cre+ mice in the presence of 0.6 mM L-serine and 0.1 mM D-serine. Error bars represent SD. Data are representative of three independent experiments. Tissues for each experiment were pooled from n = 5–8 mice per genotype.