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. 2023 Jun 29;18(7):1421–1435. doi: 10.1016/j.stemcr.2023.06.002

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© 2023 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Expression and spatial validation of epithelial and mesenchymal markers

(A) tSNE plots showing expression of (novel) markers in the epithelial (EAC3, 8) and mesenchymal (EAC1, 2, and 4) subpopulations. Expression scale is log2.

(B) Immunohistochemistry with established (WT1, AQP1) or novel markers on human fetal heart tissue. Small dashes: magnification of epicardial section of CRIP1 staining. Large dashes: magnification of epicardial section of NRK staining. Solid arrowheads: epithelial expression. Open arrowheads: mesenchymal expression. Counterstain is DAPI. Scale bar: 25 μm. See also Figure S3.