Figure 5.

CRIP1 is a marker of human fetal epicardium and is downregulated in primary human cultured epicardial cells undergoing EMT

(A) Immunohistochemistry of CRIP1 during various stages of development and in adult atrial tissue. Solid arrowheads: epithelial expression. Open arrowheads: mesenchymal expression. Counterstain is DAPI. Scale bar: 25 μm.

(B) qPCR of various epicardial and mesenchymal markers after 5 days in control conditions or after the addition of TGFβ to induce EMT. Data are presented as mean ± SEM of three independent samples. ^∗p < 0.05, ^∗∗∗p < 0.001; Student’s t test.