Figure 3.

Characterization of contractile dynamics of cardiac MTs in MToC and VMToC

(A and B) Representative images of MTs in chips on days 0, 1, 3, and 5 in MToC (A) and VMToC (B) (10×). Scale bar, 300 μm.

(C and D) Representative confocal images of sarcomeres showing hiPSC-ECs (orange, mCherry) and hiPSC-CMs (green, ACTN2) in MToC (C) and VMToC (D) (40×). White dashed box is the area that is zoomed 100×. Scale bar, 50 μm.

(E and F) Quantification of the sarcomere parameters: sarcomere length (E); sarcomere alignment index (F); error bars are shown as mean ± SD from MToC N = 3, n = 11; VMToC N = 4, n = 8; three or four independent experiments with at least eight MTs.

(G) Representative beating traces of MTs from MToC (green trace) and VMToC (orange trace).

(H–L) Quantification of the contraction parameters: contraction amplitude (H), contraction duration (I), time to peak (J), relaxation time (K), and peak-to-peak time (L) in MTs with AICS-0075 hiPSC-CMs. Error bars are shown as mean ± SD from MToC N = 3, n = 69; VMToC N = 3, n = 132; three independent experiments with 16 or 24 MTs from at least six different microfluidic channels each experiment. Student’s t test (E), Wilcoxon-Mann-Whitney test (F and H–L). ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; ns, not significant.

See also Figure S3.