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. 2023 Mar 17;7(13):3128–3139. doi: 10.1182/bloodadvances.2022009402

Table 1.

Baseline characteristics and posttransfusion Hb decrease

Case Sex Age, y Diagnosis DAT results Type of AIHA RBC units transfused Hb, g/dL LDH,
U/L
Bilirubin total, μmol/L Posttransfusion Hb decrease <1 g/dL per 24 hr
Before enrollment (without C1-INH) § On trial (with
C1-INH)
1 F 45 CAS IgG: 4+ Mixed 2 5.6 975 65 Yes Yes
APS IgM: 2+
IgA: 2+
C3d: 3+
2 F 37 CAS IgG:4+,
IgM: 2+,
Mixed 3 3.7 1118 152 No No
IBD IgA: neg
T-cell NHL, allo-SCT C3d: 3+
3 F 38 CAS IgG: 4+ Mixed 7 3.5 911 55 No data No
SLE IgM: 4+,
RA IgA: 3+
IBD C3d: 4+
ITP
4 M 69 CAS IgG: 4+ Mixed 2 5.3 559 36 No data Yes
B-cell NHL IgM: neg
IgA: neg
C3d: 3+
5 M 59 CAD IgG: neg
IgM: neg
IgA: neg
C3d: 2+
Cold 1 4.8 605 30 Yes Yes
6 F 46 CAS, allo-SCT, MF IgG: 2+
IgM: neg
IgA: neg
C3d: 3+
Mixed 23 5.3 270 53 N/A Yes
7 M 65 CAS, CLL IgG: neg
IgM: 1+
IgA: neg
C3d: 3+
Cold 8 5.5 526 96 No No
8 M 72 CAS, IgG: neg Cold 2 6.2 476 13 Yes Yes
CLL IgM: neg
IgA: neg
C3d: 4+
9 F 77 CAD IgG: neg Cold 4 6.9 570 26 Yes Yes
IgA: neg
IgM: 2+
C3d: 4+
10 M 75 CAD IgG: neg Cold 1 6.9 495 55 Yes Yes
IgM: neg
IgA: neg
C3d: 3+
Median 62 2.5 5.4 564.5 54
Response/no. of patients 5/7 7/10

Eight out of 10 patients received either a prophylactic or therapeutic dose of anticoagulation.

allo-SCT, allogeneic stem cell transplantation; APS, antiphospholipid syndrome; CAS, cold agglutinin syndrome; CLL, chronic lymphocytic lymphoma; F, female; IBD, inflammatory bowel disease; ITP, immune thrombocytopenia; neg, negative; NHL, non-Hodgkin lymphoma; M, male; MF, myelofibrosis; RA, rheumatoid arthritis; SLE, systemic lupus erythematosus.

AIHA classified as warm AIHA: DAT positive for IgG and/or IgA ± C3d when a clinically significant cold-reactive antibody has been excluded; cold AIHA: monospecific DAT strongly positive for C3d (and negative or weakly positive with IgG) and CAs are present; mixed AIHA: diagnosed in patients with a DAT positive for C3d and IgG DAT, a CA with a thermal amplitude ≥30 °C, and evidence of a warm IgG antibody by eluate.

Total in the 30 days before inclusion.

Normal values: Hb male, 13.2 to 16.6 g/dL; Hb female, 11.6 to 15 g/dL; LDH, 0 to 247 U/L; total bilirubin, 0 to 17 μmol.

§

Post hoc analysis (see “Methods”).

Per protocol analysis.

IgG weakly positive in DAT, No evidence for IgG reacting autoantibodies in the eluate.