Fig. 1.

GDF-11 downregulates CGB expression in BeWo and JEG-3 cells. A and B BeWo (A) and JEG-3 (B) cells were treated with 30 ng/mL GDF-11 (G11) for 24 h or every 24 h for 48 h. The protein levels of CGB were examined by western blot. C and D BeWo (C) and JEG-3 (D) cells were treated with 1, 5, 10, and 30 ng/mL GDF-11 every 24 h for 48 h. The protein levels of CGB were examined by western blot. The results are expressed as the mean ± SEM of at least three independent experiments. The values without a common letter are significantly different (p < 0.05)