FIG. 4.

Kinetics of proU-lacZ (A) and wcaB-lacZ (B) fusion expression in attached and free-living bacteria of adherent ompR wild-type strains (PHL1064 and PHL1036, respectively) overproducing the CsgD specific activator of the curlin-encoding csgBA operon. Specific β-galactosidase activities were measured in free-living (open squares) and sessile (filled squares) bacteria during biofilm development on petri dishes in M63 medium supplemented with ampicillin and mannitol (A) or glucose (B), as described in Materials and Methods. Results are means ± standard deviations of three independent measurements.