FIG. 6.

Kinetics of up-regulated lacZ fusion expression (strain PHL665) in attached and free-living bacteria (A) and comparison of fusion expression under standard conditions and during anaerobiosis after 28 h of biofilm cultures (B). Biofilm cultures were performed in M63-mannitol medium on petri dishes, and specific β-galactosidase activities were measured in free-living bacteria (open squares and bars) and sessile bacteria (filled squares and hatched bars) as described in Materials and Methods. Results are means ± standard deviations of three independent measurements.