TABLE 1.
E. coli K-12 strains and plasmids used
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| CSH135 | F′ lacZ::Mu cts62 Mu dX Δ(gpt-lac) his met tyr gyrA rpsL | 38 |
| DH5α | ΔlacU169 recA1 endA1 gyrA96 relA1 hsdR17 thi-1 supE44 | Laboratory collection |
| GM37 | MC4100 Φ(proU-lacZ)2(Hyb) (λp lac Mu15) | 34 |
| HYD723 | MC4100 nikA (hydC)::Mu dI(Aprlac) | 57 |
| MC4100 | araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR | Laboratory collection |
| MG1655 | λ− F− prototroph | Laboratory collection |
| MH225 | MC4100 Φ(ompC-lacZ+)10-25 | 21 |
| PHL628 | MG1655 malA-kan ompR234 | 56 |
| PHL644 | MC4100 malA-kan ompR234 | 56 |
| PHL665 | PHL644 pepT::lacZ (Mu dX) | This study |
| PHL698 | YK3421 malA-kan | This study |
| PHL699 | YK3421 malA-kan ompR234 | This study |
| PHL701 | SG20781 malA-kan ompR234 | This study |
| PHL702 | SG20781 malA-kan | This study |
| PHL744 | MC4100 malT54::Tn10 ompR234 | 56 |
| PHL789 | MC4100 pepT::lacZ (Mu dX) malT54::Tn10 | This study |
| PHL801 | MC4100 pepT::lacZ (Mu dX) ompR331::Tn10 | This study |
| PHL825 | PHL644 f92::lacZ (Mu dX) | This study |
| PHL826 | SG20781 ompR331::Tn10 | This study |
| PHL840 | MH225 malA-kan ompR234 | 56 |
| PHL861 | MC4100 f92::lacZ (Mu dX) ompR331::Tn10 | This study |
| PHL863 | GM37 malT54::Tn10 ompR234 | This study |
| PHL864 | GM37 malT54::Tn10 | This study |
| PHL865 | GM37 ompR331::Tn10 | This study |
| PHL868 | MC4100 f92::lacZ (Mu dX) malT54::Tn10 | This study |
| PHL923 | YK3421 ompR331::Tn10 | This study |
| PHL965 | HYD723 malA-kan ompR234 | This study |
| PHL1036 | SG20781 pCP994 (Apr) | This study |
| PHL1064 | GM37 pCP994 (Apr) | This study |
| SG20781 | MC4100 wcaB10 (cpsB10)::lac-Mu-immλ | 7 |
| TK821 | MC4100 ompR331::Tn10 | 21 |
| YK410 | F−araD139 ΔlacU169 rpsL thi nalA thyA pyrC46 his | 31 |
| YK3421 | YK410 fliC (hag)::Mu d(Aprlac) | 31 |
| Plasmids | ||
| pCP752 | pPH126 (51) with a 14-kb PstI fragment cloned in NsiI and harboring a genomic fragment (5 kb) with the pepT gene fused to the lacZ gene from Mu dX | This study |
| pCP832 | pBR322 with a 16-kb PstI fragment harboring a genomic fragment (7 kb) with the f92 gene fused to the lacZ gene from Mu dX | This study |
| pCP994 | pKK233-2 (2) with a 697-bp fragment containing the csgD ORF | This study |