TABLE 2.
Comparison of biofilm-regulated lacZ fusions in wild-type ompR, ompR234, and ompR null contextsa
| Strain | Relevant genotype | β-Galactosidase sp act (U/mg of protein)b |
|---|---|---|
| PHL789 | pepT-lacZ | 91 |
| PHL665 | pepT-lacZ ompR234 | 96 |
| PHL801 | pepT-lacZ ompR::Tn10 | 99 |
| PHL868 | f92-lacZ | 20 |
| PHL825 | f92-lacZ ompR234 | 17 |
| PHL861 | f92-lacZ ompR::Tn10 | 46 |
| PHL698 | fliC-lacZ | 1,537 |
| PHL699 | fliC-lacZ ompR234 | 1,309 |
| PHL923 | fliC-lacZ ompR::Tn10 | 1,375 |
| PHL702 | wcaB-lacZ | 57 |
| PHL701 | wcaB-lacZ ompR234 | 12 |
| PHL826 | wcaB-lacZ ompR::Tn10 | 469 |
| PHL864 | proU-lacZ | 57 |
| PHL863 | proU-lacZ ompR234 | 67 |
| PHL865 | proU-lacZ ompR::Tn10 | 71 |
a
Bacteria were grown at 30°C in shaken M63 liquid medium supplemented with mannitol (or glucose for strains carrying the wcaB-lacZ fusion).
b
β-Galactosidase activity was assayed at mid-log growth phase. The values are representative of those from three independent assays.