TABLE 3.
Comparison of osmoregulated fusion expression in attached and free-living bacteria grown under standard conditions or under anaerobiosis
| Fusion | Culture conditionsa | β-Galactosidase sp act (U/mg protein)b
|
|||
|---|---|---|---|---|---|
| 23 h
|
45 h
|
||||
| Free bacteria | Attached bacteria | Free bacteria | Attached bacteria | ||
| f92-lacZ | Standard | 16.8 ± 3.6 | 10.8 ± 0.9 | 20.0 ± 5.0 | 11.0 ± 0.4 |
| Anaerobiosis | NDc | ND | 15.0 ± 3.7 | 7.8 ± 0.9 | |
| ompC-lacZ | Standard | 475 ± 5 | 1,498 ± 194 | 454 ± 34 | 1,497 ± 114 |
| Anaerobiosis | 614 ± 1 | 1,340 ± 50 | 644 ± 51 | 1,530 ± 67 | |
| proU-lacZ | Standard | 7.9 ± 1.7 | 21.9 ± 4.9 | 21.0 ± 1.6 | 32.5 ± 4.7 |
| Anaerobiosis | 0 | 5.0 ± 2.4 | ND | ND | |
a
Biofilm cultures were performed in M63-mannitol medium at 30°C in petri dishes incubated in anaerobic jars (anaerobiosis) or not (standard).
b
Results are means ± standard deviations of three determinations.
c
ND, not determined.