Fig. 4.

MAL2 downregulation induces apoptosis of BC cells. A Flow cytometric analysis detects apoptosis of MDA-MB-231 and MCF-7 cells transfected with sh-NC and sh-MAL2. B AO-EB double staining evaluates apoptosis of MDA-MB-231 and MCF-7 cells transfected with sh-NC and sh-MAL2. C Western blot assay was performed to measure the expression of apoptosis-related proteins in MDA-MB-231 and MCF-7 cells after MAL2 knockdown. D The relative expression level of apoptosis-related proteins in MDA-MB-231 cells. E The relative expression level of apoptosis-related proteins in MCF-7 cells. All data are expressed as mean ± SD. * P < 0.05; ** P < 0.01;*** P < 0.001; ns, not significant