Fig. 5.

Effect of iron ions on CCCP-induced pyroptosis of Ramos cells. a FeSO₄ and CCCP were used to induce interference in Ramos cells. After 24 h, the morphology of Ramos cells was observed. The red arrow indicates the presence of swollen B cells. b Detection of Ramos cell viability by CCK8. c Detection of LDH release from Ramos cell supernatants using an LDH kit. d Detection of the levels of MCP-1 in Ramos cell supernatants via ELISA. e Detection of the expression of Ramos cell pyroptosis-related proteins by western blot. f, g After Tom20-KD by siRNA transfection, the morphology of Ramos cells was observed. The red arrow indicates the presence of swollen Ramos cells. h Detection of Ramos cell viability by CCK8 after Tom20-KD. i Detection of LDH release from Ramos cell supernatants using an LDH kit after Tom20-KD. j Detection of the levels of MCP-1 in Ramos cell supernatants via ELISA after Tom20-KD. k Western blot detection of the expression of Ramos cell pyroptosis-related proteins after Tom20-KD. All data are expressed as the mean ± SD (n ≥ 3 replicates per group). ns P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001. CCCP carbonyl cyanide m-chlorophenyl hydrazone, CCK8 cell counting kit 8, LDH lactate dehydrogenase, MCP-1 monocyte chemoattractant protein 1, ELISA enzyme-linked immunosorbent assay, Tom20-KD Tom20 knock-down, siRNA small interfering ribonucleic acid, SD standard deviation, ns not significant