Fig. 7.

Specific mechanism through which iron ions affect repair processes after SCI. a, b The spleen of mice 3 days after SCI was observed, and the spleen length and organ index of different groups were compared. c Three days after SCI, serum samples were collected to detect the concentration of iron ions. d Three days after SCI, B cell lysates were collected to detect the concentration of iron ions. e The pathological changes of the spleen were detected by HE staining. f The level of iron ion in the spleen was detected by Prussian blue staining. g The distribution of B cells in the spleen was detected by immunofluorescence. CD19⁺ was red, dapi was blue, and merge was combined. h Lymphocytes were isolated from the spleen of mice three days after SCI and detected via flow cytometry (B cells were CD19⁺, and plasma cells were CD19⁺ and CD138⁺). i, j Detection of Tom20-Bax-caspase-GSDME pathway-related protein expression in B cells by western blot. All data are expressed as the mean ± SD (n ≥ 3 replicates per group). ns P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001. SCI spinal cord injury, HE hematoxylin & eosin, CD cluster of differentiation, GSDME gasdermin E, SD standard deviation, ns not significant