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. 2023 Jul 21;21:176. doi: 10.1186/s12964-023-01196-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — HUVECs express CCDC25, as evaluated using proteomics a The quantifiable proteins differentially expressed after NET stimulation of HUVECs were identified from TMT-MS/MS spectra. b Volcanogram of changes in relative protein abundance between NET-stimulated and unstimulated groups. c The expression of CCDC25 in different endothelial cells, HUVECs, HUVECs-C, and HMVECs, as detected by western blotting. d Differences in the expression and location of CCDC25 before and after NET stimulation as observed via immunofluorescence. e The average fluorescence intensity of CCDC25 was calculated using Image J software. f The expression of CCDC25 in stage I, II, and III gastric cancer tissues was observed by immunohistochemical staining. g The area covered by CCDC25 expression in immunohistochemical staining. Magnification, × 20; scale bars: 50 μm; red, CCDC25; green, CD31; blue, DAPI. All values are presented as means ± standard deviations. **p < 0.01; ****p < 0.0001 CCDC25, coiled-coil domain containing 25; HUVEC, human umbilical vein endothelial cell; HMVEC, human pulmonary microvascular endothelial cell; NET, neutrophil extracellular trap; HUVEC-C, human pulmonary microvascular endothelial cell line