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. 2023 Jul 21;25:86. doi: 10.1186/s13058-023-01684-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Characterization of primary culture fibroblasts. Immunofluorescence using antibodies directed to specific markers for epithelial cells (panCK, red fluorescence), fibroblast intermediate filament (VIM, red fluorescence), myofibroblasts (ASMA, red fluorescence), fibroblast-associated protein (FAP, green fluorescence), and chemotactic receptor (CD10, green fluorescence; GPR77, red fluorescence) of different primary culture fibroblasts and normal fibroblasts (HDF and BNF). The nuclei were counterstained with Hoechst. All images were captured at 200 × magnification, scale bars = 50 μm. NFs, normal fibroblasts; HDF, human dermal fibroblast; BNF, breast normal fibroblast; CAFs, cancer-associated fibroblasts; panCK, pan-cytokeratin; VIM, vimentin; ASMA, alpha-smooth muscle actin; FAP, fibroblast activation protein; CD10, a cluster of differentiation 10; GPR77, G protein-coupled receptor 77