FIG. 1.

Agarose gel electrophoresis of plasmids isolated from various clones. (A) Ethidium bromide-stained gel. (B) Southern blot hybridized with probes containing the hdlIVa gene. (C) Southern blot hybridized with probes containing the chd1 gene. Lanes 1 to 3, 5, and 6 contain plasmid DNAs isolated from various clones and digested with EcoRI. Lane 7 contains a plasmid bearing the hdlIVa gene in a 1.6-kb EcoRI fragment. Lane 4 contains a molecular weight (MW) ladder (lambda DNA cut with HindIII and EcoRI). Lane 8 contains an MBA4 total DNA digested with EcoRI.