Fig. 1.

Decreased SIRT3 expression in the hippocampus of diabetic mice and HG-exposed SH-SY5Y cells. a, b Western blot analysis showing SIRT3 protein expression in hippocampal tissue. N = 3 mice per group. c, d Western blot analysis of SIRT3 protein expression in SH-SY5Y cells. GAPDH was used as the loading control. N = 3. e, f Western blot analysis of SIRT3 protein expression in cytoplasmic and mitochondrial fractions extracted from SH-SY5Y cells. Cyto, cytoplasm; Mito, mitochondria. COXIV served as the mitochondrial marker and GAPDH served as the cytoplasmic marker. N = 3. Data were expressed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001