Fig. 3.

SIRT3 rescues mitochondria Ca²⁺-induced mitochondrial dysfunction and apoptosis under HG condition. a, b Representative flow cytometry histograms and quantitative analysis of Rhod2-positive SH-SY5Y cells. Rhod-2 AM staining was performed to measure mitochondrial Ca²⁺ levels. N = 3. c, d Representative flow cytometry histograms and quantitative analysis of MitoSOX-positive cells in SH-SY5Y cells. Rhod-2 AM staining was used to detect mitochondrial ROS (mtROS). N = 3. e, f Representative confocal imaging of mitochondrial membrane potential (MMP) using TMRM staining. Analysis of MMP by measuring TMRM fluorescence signal. N = 4. g The viability of cells was detected by CCK8 assay. N = 3. h, i Western blot analysis of pro- and anti-apoptotic proteins. cCaspase-3, Cleaved Caspase-3. β-actin was used as the loading control. N = 3. Data were expressed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001