Fig. 4.

SIRT3 prevents HG-induced MAM formations. a Representative confocal images showing the co-localization of ER (ER-Tracker Blue-White DPX staining) and mitochondria (Mito-Tracker Red CMXRos staining). b, c Mander’s overlap coefficients based on confocal imaging. N = 20 images for each group. d Representative electron micrograph images showing the interactions between ER and mitochondria, scale bar = 100 nm. e, f Quantitative analysis of the distance between the mitochondria and ER (e) and the contact length (f), N ≥ 100 contacts from 25 images for each group. g Representative electron micrographs of ER-mitochondria contacts in the hippocampus, scale bar = 100 nm. h, i Quantitative analysis of the distance between the mitochondria and ER (h) and the length of contacts (i). N ≥ 100 contacts from 4 mice per group. The distance between the mitochondria and ER are presented as the median and range (25th–75th percentile), the contact length are presented as mean ± SEM and other data are presented as the mean ± SD, **p < 0.01, ****p < 0.0001