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. 2023 Jul 22;9:16. doi: 10.1186/s40851-023-00215-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Day length (top), water temperature (middle), and gonadal development of the Higashidori and Hanamaki populations (bottom) at the Hakodate outdoor experimental field. The raw data of the day length and the temperature and the GSI values are shown in Tables S8 and S9. (Bottom) Mean ± SEM, n = 4–8. **p < 0.01, ***p < 0.001 by Dunnett’s test (vs. Gonadosomatic index on April 5). The raw statistical results of one-way ANOVA and Dunnett’s post hoc tests are shown in Tables 4 and 5