Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Restriction enzyme cleavage of ParB-linearized pUC19 DNA. Supercoiled DNA was untreated (lanes 1 to 4) or treated with ParB (lanes 5 to 8) or with EcoRI (lanes 9 to 11). Samples were then analyzed without an additional digestion (lanes 1 and 5) or after digestion with SspI (lanes 2, 6, and 10), DraI (lanes 3, 7, and 11), or EcoRI (lanes 4 and 8). Agarose gel electrophoresis was carried out as described in Materials and Methods.
