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. 1999 Oct;181(19):6010–6018. doi: 10.1128/jb.181.19.6010-6018.1999

FIG. 5.

FIG. 5

Primer extension of ParB-treated supercoiled DNA. (A) pUC19 DNA was treated with FspI and AlwNI (lanes 1 and 4) or with ParB to produce open circular (oc) (lanes 2 and 5) or linear (lanes 3 and 6) DNA. Primer extension was then performed with radiolabeled primers against the top strand (lanes 1 to 3) and against the bottom strand (lanes 4 to 6) of the treated pUC19. The hatched boxes indicate the position of the AT-rich region. (B) Results of primer extension performed on the top strand of the FspI-AlwNI fragment of pUC19 and on ParB-treated open circular and linear DNAs with primer 1293TSpUC (right panel) and results of sequencing with the same primer (left panel). (C) Results of primer extension performed on the bottom strand of the FspI-AlwNI fragment of pUC19 and on ParB-treated open circular and linear DNAs with primer 1484BSpUC (right panel) and sequencing of this region with the same primer (left panel).