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Exonuclease assay. A 20-bp double-stranded DNA fragment was 5′ radiolabeled on one end and then treated with 0.01, 0.1, or 1 U of exonuclease III (lanes 2 to 4, respectively); with 0.1, 1, or 2 U of the T7 gene 6 exonuclease (lanes 5 to 7, respectively); or with 0.1, 1, 10, 20, or 40 ng of purified ParB (lanes 8 to 12, respectively). The positions of AMP, ADP, and ATP are indicated.
