Figure 5:

Characterization of activity peaks in pdf⁰¹ mutants. (A) Representative activity profiles of four males each of w¹¹¹⁸ controls (top) and pdf⁰¹ mutants (bottom) overlaid with SG-filtered waveforms show advanced evening peaks and reduced morning peaks in pdf⁰¹ flies. We quantified phases and heights of both morning and evening activity peaks and found that phase of morning peak is significantly delayed in pdf⁰¹ flies (B; W = 264.5; p = 0.0009), and the height of morning peak is significantly lower compared to w¹¹¹⁸ flies (C; W = 879; p = 1.581e-07). On the other hand, the phase of evening peak is significantly advanced (D; W = 794.5; p = 0.0001521) and its height significantly lower (E; W = 800; p = 6.718e-05) in pdf⁰¹ mutants compared to w¹¹¹⁸ flies. Comparisons were done using Mann-Whitney U tests implemented on R. * < 0.05, ** < 0.01, *** < 0.001, and NS (Not Significant).