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. Author manuscript; available in PMC: 2023 Jul 22.
Published in final edited form as: ACS Synth Biol. 2021 Sep 1;10(9):2359–2370. doi: 10.1021/acssynbio.1c00258

Figure 3.

Figure 3.

Root mean square fluctuation (RMSF) analysis of CHMO DTNP. Results are plotted as the difference in RMSF between DTNP (with NADH bound) and WT (with NADH or NADPH bound, respectively). The control loop is found to have greater stability with DTNP:NADH, comparable to the native condition of WT:NADPH. The inactive pairing of WT:NADH exhibits high flexibility at the control loop.