Table 1.

Kinetic Characterization of CHMO Variants^a

	NADH			NADPH
variant	kcat (s−1)	KM (mM)	kcat/KM (mM−1 s−1)	kcat (s−1)	KM (mM)	kcat/KM (mM−1 s−1)
WT	n.d.	n.d.	0.18 ± 0.01	13.5 ± 0.55b	0.037 ± 0.01b	370 ± 85b
DTN	n.d.	n.d.	0.21 ± 0.03	n.d.	n.d.	3.39 ± 0.03
DTNP	2.46 ± 0.36	2.79 ± 0.43	0.88 ± 0.01	n.d.	n.d.	1.45 ± 0.12
DTNPY	5.08 ± 0.48	2.81 ± 0.21	1.81 ± 0.03	n.d.	n.d.	1.28 ± 0.04

^an.d. Individual k_cat or K_M were not determined because the enzyme cannot be saturated with the cofactor concentrations tested (up to 4 mM). In which case, the catalytic efficiency (k_cat/K_M) was determined by fitting the modified Michaelis–Menten equation ν₀ = V_max [S]/K_M

^bValues taken from Maxel et al.²⁰