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. Author manuscript; available in PMC: 2023 Jul 22.
Published in final edited form as: Methods Appl Fluoresc. 2023 Jan 31;11(1):10.1088/2050-6120/acb2b4. doi: 10.1088/2050-6120/acb2b4

Table 1.

Relative change (Rc) in PIC emission and estimated PIC fluorescence QY (Φ) utilizing 523 nm excitation and in AF emission and QY using a 647 nm excitation.

Sample PIC (523 nm excitation) AF (647 nm excitation)
Rc (%) Φ (%) Rc (%) Φ (%)
AT 15.4 ± 1.5 0.23 ± 0.03 −80.8 ± 2.6 12.8 ± 4.6
GC −25.7 ± 0.6 0.15 ± 0.02 −84.6 ± 1.4 12.1 ± 2.5
dsDNA −40.8 ± 1.1 0.12 ± 0.02 −95.5 ± 0.2 4.8 ± 0.9
ssDNA −43.7 ± 0.6 0.10 ± 0.01 −95.9 ± 0.3 6.1 ± 3.6
PIC (monomer) −74.3 ± 0.2 0.04 ± 0.01 — —
J-bit (Reference) — 0.18 ± 0.03 — —
Free AF — — −2.6 ± 0.7 33.0 ± 3.3
*

DNA sequences and structures are available in figures S1–S4.