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(A) Structure of cholesteryl ester. (B) Structure of wax ester. The protons shown exhibit a resonance near 4.0 ppm in C. (C, D) A typical NMR spectrum of meibum from a 31-year-old male White donor. The numbering is associated with cholesteryl ester carbons numbered in A. The resonance associated with cholesterol number C21 is a doublet.
