Table 3.

Post Hoc Analyses of the Cholesteryl Ester/Wax Ester Molar Ratio in Human Meibum

Cohort	Molar Ratio of CE/WE* (Samples With Measurable CE)	Molar Ratio of CE/WE* (All Samples)	Molar Ratio of CE/WE†
Non-MGD	0.51 ± 0.03 (13)	0.28 ± 0.06 (22)	0.38 ± 0.05 (22)
Mild-to-moderate MGD	0.36 ± 0.05 (8)	0.14 ± 0.04 (21)	0.25 ± 0.03 (21)
Severe MGD	0.23 ± 0.04 (5)	0.06 ± 0.03 (21)	0.21 ± 0.03 (21)
All MGD (mild-to-moderate + severe)	0.31 ± 0.04 (13)	0.10 ± 0.03 (42)	0.23 ± 0.02 (42)
P, non-MGD vs mild-to-moderate MGD	0.013	0.06	0.44
P, mild-to-moderate vs severe MGD	0.096	0.12	0.39
P, non-MGD vs severe MGD	<0.0001	0.002	0.0007
P, non-MGD vs all MGD	0.0005	0.0038	<0.0001

CE, cholesteryl ester; WE, wax ester.

The post hoc analyses used a curve-fitting algorithm to more accurately integrate the 4.0 ppm triplet peak. Data are mean ± standard error of the mean. The number of samples is shown in parentheses.

^*Calculated using the 4.6 and 4.0 ppm resonances, as in the initial analysis.

^†Calculated using the 1.0, 0.63 and 4.0 ppm resonances; all samples had measurable CE.