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Nondenaturing electrophoresis of periplasmic extracts (50 μg of protein) of R. sphaeroides f. sp. denitrificans wild type (WT) and MS523 mutant grown under phototrophic conditions in the presence (+) or absence (−) of 20 mM nitrate. Gels were stained for nitrate (A), nitrite (B), and N2O (C) reductase activities with dithionite-reduced methyl viologen as the electron donor or with Coomassie R250 for protein detection (D).
