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Nondenaturing electrophoresis of periplasmic extracts (50 μg of protein) of the MS523 mutant containing pRK415 or pMS538 in trans. Cells were grown under phototrophic conditions in the presence (+) or absence (−) of 20 mM nitrate. Gels were stained for nitrate (A), nitrite (B), and N2O (C) reductase activities with dithionite-reduced methyl viologen as the electron donor.
