Table 1.
Five extraction solutions prepared by combinations of chemicals and reagents
| 1, 2Extraction solution | Description |
|---|---|
| PB | 0.1 M phosphate buffer (pH 7.5) |
| PS | PB + 1.5 g/100 mL SDS |
| PD | PB + 0.05 M DDT |
| PU | PB + 8 M urea |
| PSDU | PB + 1.5 g/100 mL SDS + 0.05 M DDT + 8 M urea |
1 PB represents phosphate buffer; PS represents PB + sodium dodecyl sulfate (SDS); PD represents PB + dithiothreitol (DTT); PU represents PB + urea; PSDU represents PB + SDS + DTT + urea. 2 PB with a neutral pH was used as the control extraction solution to dissolve native protein. SDS, DTT, and urea were used to destroy hydrophobic interactions, disulfide bonding, and hydrogen bonding, respectively.