Fig. 3.

Expression of glucose transporter Glut-1 and glucose uptake by spleen CD4+ T cells from uninfected or from S. aureus-infected mice upon stimulation with anti-CD3/anti-CD28 antibodies. a Flow cytometry histograms showing the levels of intracellular Glut-1 expression in CD4+ T cells in spleen cells isolated from either uninfected (left panel) or S. aureus-infected (right panel) mice cultured for 24 h in the presence (red histogram) or absence (black histogram) of anti-CD3/anti-CD28 antibodies. Quantification of intracellular Glut-1 expression levels in the different groups is shown in b. c Flow cytometry histograms showing the kinetic of glucose uptake in CD4+ T cells in spleen cells isolated from either uninfected (upper panel) or S. aureus-infected (lower panel) mice unstimulated (black histograms) or stimulated for 24 h (pink histograms), 48 h (green histograms) or 72 h (blue histograms) with anti-CD3/anti-CD28 antibodies. Glucose uptake was determined using 2-NBDG. Quantification of glucose uptake in the different groups is shown in d. e percentage of divided CD4+ T cells in spleen cells from either uninfected (black bars) or S. aureus-infected (white bars) mice at 72 h upon stimulation with anti-CD3/anti-CD28 antibodies in the presence of either 10 mM, 50 mM or 100 mM glucose. f Frequencies of CD4+ T cells expressing IL-2 (left panel) or IFN-γ (right panel) within the spleen cell population isolated from uninfected (black bars) or S. aureus-infected (white bars) mice at 72 h upon stimulation with anti-CD3/anti-CD28 antibodies in the presence of either 10 mM, 50 mM or 100 mM glucose. Each bar shows the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001