Fig. 7.

NAD+/NADH ratio in stimulated spleen CD4+ T cells from uninfected or S. aureus-infected mice. a NAD+/NADH ratio in CD4+ T cell isolated from cultured spleen cells from either uninfected (black symbols) or S. aureus-infected mice in the presence (white symbols) or absence (grey symbols) of 200 µM NR and stimulated with anti-CD3/anti-CD28 antibodies. Each symbol shows the mean ± SD of three independent experiments. b Flow cytometry histograms showing proliferation of CD4+ T cell in spleen cells isolated from either uninfected (left histograms) or S. aureus-infected (right histograms) mice at 72 h upon stimulation with anti-CD3/anti-CD28 antibodies in the absence (upper histogram) or presence (lower histograms) of NR (200 µM). The percentage of divided CD4+ T cells in each group is shown in c. Each bar shows the mean ± SD of five independent experiments. *p < 0.05, **p < 0.005, ***p < 0.001