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. 2023 Apr 6;36(3):243–254. doi: 10.1007/s00497-023-00462-x

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Fig. 6 — Developmental analysis of GC shape. The dimensions of GCs at three stages of development (EBC, MBC and LBC) were analysed from WT pollen and from duo1 pollen segregating in homozygous TET11-GFP/HTR10:H2B-RFP marker plants. Box plots show the distribution of GC dimension parameters for major axis (a), minor axis (b), aspect ratio (c) and circularity (d) calculated in ImageJ. Centre lines show medians and crosses means in the boxed interquartile range; whiskers extend 1.5 times the interquartile range. Letters above whiskers indicate significantly different means (P < .05) determined with One-Way ANOVA and Tukey’s HSD Test for multiple comparisons. EBC, early bicellular pollen; MBC, mid-bicellular pollen; LBC; late bicellular pollen