Skip to main content
. 1999 Oct;181(19):6033–6041. doi: 10.1128/jb.181.19.6033-6041.1999

FIG. 3.

FIG. 3

Western blot (immunoblot) analysis of HemA[WT] and HemA[KK] expressed from a gene carried in a single copy in the E. coli chromosome. Cultures were grown to an OD600 of 0.4 in minimal MOPS-glycerol medium with 2 μM ALA and tryptophan (and ampicillin where applicable). They were processed for immunoblotting with anti-HemA MAb H23. Lanes: a, E. coli TE3057 hemA8 trp::put::hemA+; b, E. coli TE7202 hemA8/pTE570 (vector); c, TE7518 hemA8 trp::put::hemA[WT]; d, TE7519 hemA8 trp::put::hemA[KK].