TABLE 1.
Bacterial strains used in this study
| Strain | Genotype | Source or reference |
|---|---|---|
| S. typhimurium | ||
| LT-2 | Wild type | Lab collection |
| TE277 | zde-1858::Tn10d-Tet (75% linked to hemA) | 11 |
| TE299-1 | ΔhemL376 | 11 |
| TE315 | TR5877 = hsdL6 hsdSA29 (rLT− mLT+ rS− mS+) metA22 metE551 ilv-452 trpB2 xyl-404 rpsL120 (Strr) H1-b H2-e,n,x (Fels2−)nml | B. A. D. Stocker |
| TE518 | hemA60 recA1 | Lab collection |
| TE768 | araC1 DUP[(cob-4)*Tn10*(zdd-1852)] | 11 |
| TE1277 | hsdL (r− m+) galE542 (Fels?) | Lab collection |
| TE1303 | hemE1 env-53 | Lab collection |
| TE2279 | TE315 zde-1858::Tn10d-Tet hemA427 (Am) srl-203::Tn10d-Cam recA1 | Lab collection |
| TE3345 | TE315 putPA1303::Kanr-hemA (MluI fill-in)-lac [pr] (codon 416) | This study |
| TE3347 | TE315 putPA1303::Kanr-hemA (MluI fill-in)-prfA-lac [pr] (codon 6) | This study |
| TE3726 | LT2/pTE367 (AmprprfA+) | 12 |
| TE4351 | pyrD121 Δ(putPA)521/F+zzf-6807::[Tn10d-putPA1302::Cam] | 10 |
| TE7590 | pyrD121 Δ(putPA)521/F+zzf-6807::[Tn10d-putPA1303::Kanr-hemA (MluI fill-in)-lac [pr] (codon 416)] | P22.TE3345 × TE4351 |
| TE7591 | pyrD121 Δ(putPA)521/F+zzf-6807::[Tn10d-putPA1303::Kanr-hemA (MluI fill-in)-prfA-lac [pr] (codon 6)] | P22.TE3347 × TE4351 |
| TE7619 | hemA60 recA1/F+zzf-6807::[Tn10d-putPA1303::Kanr-hemA[WT]lac [pr] (codon 416)] | This study |
| TE7620 | hemA60 recA1/F+zzf-6807::[Tn10d-putPA1303::Kanr-hemA[WT]prfA-lac [pr] (codon 6)] | This study |
| TE7621 | hemA60 recA1/F+zzf-6807::[Tn10d-putPA1303::Kanr-hemA[KK]lac [pr] (codon 416)] | This study |
| TE7622 | hemA60 recA1/F+zzf-6807::[Tn10d-putPA1303::Kanr-hemA[KK]prfA-lac [pr] (codon 6)] | This study |
| TE7676 | hsdL (r− m+) galE542 (Fels?) DUP[(cob-4)*Tn10*(zdd-1852)] | P22.TE768 × TE1277 |
| TE7677 | hsdL (r− m+) galE542 (Fels?) DUP[(cob-4)*Tn10*(zdd-1852 hemA743::Kan(MluI)] | P1.TE6730 × TE7676 |
| TE7678 | hemA743::Kan (MluI)/pTE367 | P22.TE7677 × TE3726 |
| TE7679 | hemA744[KK]/pTE367 | P22.TE7622 × TE7678 |
| TE7691 | hemA60 recA1/F+zzf-6807::[Tn10d-putPA1303::Kanr-hemA[AA]lac [pr] (codon 416)] | This study |
| TE7693 | hemA60 recA1/F+zzf-6807::[Tn10d-putPA1303::Kanr-hemA[AA]prfA-lac [pr] (codon 6)] | This study |
| TE7695 | hemA60 recA1/F+zzf-6807::[Tn10d-putPA1303::Kanr-hemA[N-myc]-lac [pr] (codon 416)] | This study |
| TE7696 | hemA60 recA1/F+zzf-6807::[Tn10d-putPA1303::Kanr-hemA[N-myc]-prfA-lac [pr] (codon 6)] | This study |
| TE7697 | zde-1858::Tn10d-Tet hemA744[KK]/pTE367 | P22.TE277 × TE7679 |
| TE7700 | zde-1858::Tn10d-Tet hemA744[KK] | P22.TE7697 × LT2 |
| TE7701 | zde-1858::Tn10d-Tet hemA+ | P22.TE7697 × LT2 |
| TE7725 | hemEl env-53 zde-1858::Tn10d-Tet hemA744[KK] | P22.TE7700 × TE1303 |
| TE7726 | hemE1 env-53 zde-1858::Tn10d-Tet hemA[WT] | P22.TE7700 × TE1303 |
| TE7727 | ΔhemL376 zde-1858::Tn10d-Tet hemA744[KK] | P22.TE7700 × TE299-1 |
| TE7728 | ΔhemL376 zde-1858::Tn10d-Tet hemA[WT] | P22.TE7700 × TE299-1 |
| E. coli | ||
| LMG194 | K-12 F− λ− ΔlacX74 galE galK thi rpsL ΔphoA (PvuII) | L. Guzman |
| TE2331 | K-12 F− λ− IN(rrnD-rrnE) ΔlacX74 rpsL galK2 | R. Menzel |
| TE3057 | K-12 F− λ−araD139 ΔlacX74 galU galK hsdR (Strr) recD1903::mini-Tet hemA8 trpDC700::putPA1304::[′prsA-uorf-hemA-prfA-dorf-kdsA′] | 12 |
| TE2640 | K-12 F− λ− IN(rrnD-rrnE) ΔlacX74 rpsL galK2 hemA8 | 12 |
| TE4498 | MC4100 clpP1::Cm | 37 |
| TE6730 | K-12 F− λ−araD139 ΔlacX74 galU galK hsdR (Strr) recD1903::mini-Tet hemA8 trpDC700::putPA1304::[′prsA-uorf-hemA743::Kan (MluI)-prfA-dorf-kdsA′] | This study |
| TE7054 | K-12 F− λ− IN(rrnD-rrnE) ΔlacX74 rpsL galK2 hemA8 leu::Tn10 Δara714 | P1.LMG194 × TE2640 |
| TE7023 | MC4100 lon-146::ΔTn10 clpP1::Cm | 37 |
| TE7034 | MC4100 lon-146::ΔTn10 | 37 |
| TE7202 | TE7054/pTE570 (PBAD vector) | 7 |
| TE7207 | TE7054/pTE694 (PBAD-hemA[WT]) | This study |
| TE7420 | TE7054/pTE713 (PBAD-hemA[KK]) | This study |
| TE7518 | K-12 F− λ−araD139 ΔlacX74 galU galK hsdR (Strr) recD1903::mini-Tet hemA8 trpDC700::putPA1304::[′prsA-uorf-hemA[WT]-prfA-dorf-kdsA′] | This study |
| TE7519 | K-12 F− λ−araD139 ΔlacX74 galU galK hsdR (Strr) recD1903::mini-Tet hemA8 trpDC700::putPA1304::[′prsA-uorf-hemA[KK]-prfA-dorf-kdsA′] | This study |
| TE7661 | K-12 F− λ−araD139 ΔlacX74 galU galK hsdR (Strr) recD1903::mini-Tet hemA8 trpDC700::putPA1304::[′prsA-uorf-hemA[AA]-prfA-dorf-kdsA′] | This study |
| TE7663 | K-12 F− λ−araD139 ΔlacX74 galU galK hsdR (Strr) recD1903::mini-Tet hemA8 trpDC700::putPA1304::[′prsA-uorf-hemA[N-myc]-prfA-dorf-kdsA′] | This study |
| TE7706 | MC4100 clpP1::Cm/ F+zzf-6807::[Tn10d-putPA1303::Kanr-hemA[WT]-lac [pr] (codon 416)] | This study |
| TE7707 | MC4100 lon-146::ΔTn10 clpP1::Cm/ F+zzf-6807::[Tn10d-putPA1303::Kanr-hemA[WT]-lac [pr] (codon 416)] | This study |
| TE7708 | MC4100 lon-146::ΔTn10/F+zzf-6807::[Tn10d-putPA1303::Kanr-hemA[WT]-lac [pr] (codon 416)] | This study |
| TE7709 | MC4100 clpP1::Cm/F+zzf-6807::[Tn10d-putPA1303::Kanr-hemA[KK]-lac [pr] (codon 416)] | This study |
| TE7710 | MC4100 lon-146::ΔTn10 clpP1::Cm/F+zzf-6807::[Tn10d-putPA1303::Kanr-hemA[KK]-lac [pr] (codon 416)] | This study |
| TE7711 | MC4100 lon-146::ΔTn10/F+zzf-6807::[Tn10d-putPA1303::Kanr-hemA[KK]-lac [pr] (codon 416)] | This study |
| TE7712 | MC4100 clpP1::Cm/F+zzf-6807::[Tn10d-putPA1303::Kanr-hemA[AA]-lac [pr] (codon 416)] | This study |
| TE7713 | MC4100 lon-146::ΔTn10 clpP1::Cm/F+zzf-6807::[Tn10d-putPA1303::Kanr-hemA[AA]-lac [pr] (codon 416)] | This study |
| TE7714 | MC4100 lon-146::ΔTn10/F+zzf-6807::[Tn10d-putPA1303::Kanr-hemA[AA]-lac [pr] (codon 416)] | This study |