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. 2023 Jul 22;13:11842. doi: 10.1038/s41598-023-39095-5

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Relative gene expression after the cold storage period for colonies placed in the cold storage unit (“CSU”) and those kept outside the cold storage unit in ambient conditions (“out”) for both years of the study. (A) vitellogenin; (B) vitellogenin-like-A; (C) catalase; (D) superoxide dismutase; (E) heat shock protein 70; and (F) heat shock protein 90. “CSU” indicates colonies that were placed in the cold storage unit and “out” indicates colonies that remained outdoors. All comparisons between “Oct 2020” and “Oct 2021” were significant with a significant effect size.