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. 2023 Jul 22;13:11842. doi: 10.1038/s41598-023-39095-5

Table 3.

F ratios and P values for cold storage (+ or −), year (2020 or 2021), and their interactions in ANOVAs for individual level response variables. Response variables and covariates had been transformed as log values. “Cold storage” indicates exposure to cold storage; “Pre-treat values” indicates the values obtained from sample prior to cold storage in September and were used as covariates; “VG” indicates vitellogenin; “Sup. dismut.” indicates superoxide dismutase; “HSP” indicates heat shock protein. Effects with significant P values are in bold.

Response variable Cold storage Year Cold storage × Year Pre-treat values
F P F P F P F P
MDA 5.77 0.0187 0.96 0.3304 3.17 0.0788 0.36 0.5484
Carbonyl 2.92 0.0917 11.95 0.0009 2.99 0.0879 0.62 0.4337
Vitellogenin 0.57 0.4509 22.63 < 0.0001 0.03 0.8554 0.00 0.9827
VG-like A 4.81 0.0314 38.34 < 0.0001 0.05 0.8251 1.79 0.1856
Catalase 2.05 0.1564 55.61 < 0.0001 0.67 0.4141 0.82 0.3688
Sup. dismut 0.02 0.8866 48.12 < 0.0001 1.71 0.1951 0.17 0.6847
HSP 70 1.15 0.2872 7.94 0.0073 5.91 0.0174 0.31 0.5816
HSP 90 1.28 0.2623 25.32 < 0.0001 0.62 0.4324 0.04 0.8455