Table 5.
Primers used for gene expression analyses. “Annealing temp.” is the annealing temperature.
| Gene (accession number) | Forward 5′–3′ | Reverse 5′–3′ | Annealing temp. (°C) | References |
|---|---|---|---|---|
| Actin (XM_623378) | TGCCAACACTGTCCTTTCTG | AGAATTGACCCACCAATCCA | 55.0 | 33 |
| vitellogenin (vg) (AJ517411) | GTTGGAGAGCAACATGCAGA | TCGATCCATTCCTTGATGGT | 57.5 | 34 |
| vg-like-A XM_001121939.3 | GTTTATGACGAAAATGGACACCT | TGAACAGTTTCCTCGTGAGTT | 57.5 | 34 |
| Catalase (NM_001178069) | TTCTACTGTGGGTGGCGAAAG | GTGTGTTGTTACCGACCAAATCC | 60.0 | 35 |
| Sup. dismut (NM_001178027) | TCAACTTCAAGGACCACATAGTG | ATAACACCACAAGCAAGACGAG | 60.0 | 35 |
| HSP70 (GB19503) | GACGCGGGAGCGATAGCAGG | AAGCCATAAGCAATCGCCGCC | 60.0 | 17 |
| HSP90 (GB14758) | ATGCCGGAGGACGTCACCAT | TTGTGCAATTTCAGCTTGGAAAGCG | 56.0 | 17 |
| DWV-A (AY292384.1) | GAG ATT GAA GCG CAT GAA CA | TGA ATT CAG TGT CGC CCA TA | 54.0 | 36 |
| DWV-B (AY251269) | CTG TAG TTA AGC GGT TAT TAG AA | GGT GCT TCT GGA ATA GCG GAA | 55.0 | 37 |