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. Author manuscript; available in PMC: 2024 Aug 1.
Published in final edited form as: Biol Psychiatry. 2023 Feb 1;94(3):249–261. doi: 10.1016/j.biopsych.2023.01.022

Figure 4. Exogenous neurosteroid-analog SGE-516 promoted restoration of endogenous allopregnanolone in vivo and in vitro through 5α-reductases type 1 and 2.

Figure 4.

LC-MS/MS measurement of allopregnanolone levels from control, CUS, and CUS+SGE-516 mice. (A) plasma, control n=10, CUS n=13, CUS+SGE-516 n=14 (B) whole brain, control n=6, CUS n=4, CUS+SGE-516 n=8 and (C) BLA samples, control n=4, CUS n=5, CUS+SGE-516 n=8. (D) qRT-PCR measurement of BLA mRNA levels of Srd5a1 and Srd5a2 normalized to β-actin and control levels, control n=16; CUS n=20; CUS+SGE-516 n=16 pooled BLA samples from 4 mice per experiment. (E) Representative images of Srd5a1 and Srd5a2 expressing cell lines showing cell confluency by DAPI staining left, and Srd5a1 and Srd5a2 GFP tag right. Quantification of NADP assay readout for luminescence from (F) Srd5a1 and (G) Srd5a2 expressing cell lines following application of control, progesterone, and SGE-516 treatments.