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. Author manuscript; available in PMC: 2024 Aug 1.
Published in final edited form as: Biol Psychiatry. 2023 Feb 1;94(3):249–261. doi: 10.1016/j.biopsych.2023.01.022

Figure 5. Knockdown of Srd5a1 and Srd5a2 in the BLA induced behavioral deficits.

Figure 5.

(A) (left) A schematic of the sgRNA construct for the knockdown of Srd5a1 and Srd5a2 in the BLA of constitutive Cas9 mice and the experimental timeline (right). The average expression of Srd5a1 and Srd5a2 mRNA in the BLA of sg5α1/2mice decreased compared to controls measured using qRT-PCR normalized to β-actin levels (B) control n=15, sg5α1/2 n=8. (above) Representative heat maps of movement of control and sgSrd1/2 mice in the light/dark box test. (below) Knockdown of 5α1/2 increased avoidance behaviors, evident from a decrease in the amount of time spent in the light chamber of the light/dark box with no change in basic movements (C) control n=15, sg5α1/2 n=8. Knockdown of 5α1/2 in the BLA increased stress-induced helplessness, evident by a decreased latency to immobility and an increased total time spent immobile during the tail suspension test (D) control n=14, 5α1/2 n=9.(E) Summary of behavioral outcomes for transparency across behavioral tests. * denotes p < 0.05, **p<0.01, ***p<0.001, ****p<0.0001 using an unpaired t-test.