KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-cFos | Sigma | Lot No. 1117M837V |
| Rabbit anti-δ-GABAAR | PhosphoSolutions | Cat No. 868A-GDN |
| Mouse anti-PV | Sigma | Cat No. P 3088 |
| rabbit anti-GFP | Cell Signaling | Lot No. 2956 |
| Monoclonal β-tubulin | Sigma | Cat No. T8328 |
| Goat anti-Rabbit Alexa Fluor 488 | ThermoFisher Scientific | Cat No. A-11008 |
| Biotinylated goat anti-mouse Alexa 647 | ThermoFisher Scientific | Cat No. A28181 |
| Donkey anti-rabbit IgG HRP conjugated | Fisher Scientific | Cat No. 45–000-683 |
| Sheep anti-mouse IgG HRP conjugated | Fisher Scientific | Cat No. 45–000-692 |
| Vectashield hardset antifade mounting medium with DAPI | Vector Labs | Cat No. H-1500–10 |
| Amersham ECL anti-mouse IgG, peroxidase-linked whole antibody | GE Healthcare | Cat No. NA931 |
| Cell Line | ||
| Srd5a1 construct | Origene | Cat No. MR203282 |
| Srd5a1 construct | Origene | Cat No. MR223814 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| DAB based HRP substrate | Vector Laboratories | SK-4100 |
| PhosSTOP phosphatase inhibitor tablets | Roche | SK-4906837001 |
| cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail | Roche | SK-11836170001 |
| Pierce™ ECL Western Blotting Substrate | ThermoFisher Scientific | Cat No. P132106 |
| SGE-516 Rodent Chow | Teklad | Lot No. 21082311i |
| Progesterone | ||
| SGE-516 | ||
| Allopregnanolone internal standard | Tocris | Cat No. 5532 |
| Allopregnanolone (mass spec reference material) | Tocris | Cat No. 3653 |
| Formic Acid | Millipore-Sigma | Cat No. A695076 |
| Acetonitrile | Millipore-Sigma | Cat No. 39998 |
| Methanol | Thermo-Fisher Scientific | Cat No. A456 |
| Chlorobutane | Merck KGaA | Cat No. 8.01640.1000 |
| Critical Commercial Assays | ||
| VECTASTAIN® Elite ABC-HRP Kit, Peroxidase (Rabbit IgG) | Vector Laboratories | PK-6101 |
| SuperScript III Platinum Sybr Green (one step qRT PCR kit) | Invitrogen | Lot No. 2028735 |
| NADP/NADPH-Glo™ Assays | Promega | Cat No. G9081 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57Bl/6J | The Jackson Laboratory (JAX) | Stock: #000664 |
| Mouse: Rosa26-LSL-Cas9 | The Jackson Laboratory (JAX) | Stock # 024857 |
| Mouse: constitutively expressing Cas9 | The Jackson Laboratory (JAX) | Stock # 024858 |
| Mouse: PV-Cre | The Jackson Laboratory (JAX) | Stock: # 012358 |
| Recombinant DNA | ||
| Single guide RNA for Gabrd | Kong Lab-Boston Children's Hospital | |
| Single guide RNA for Srd5a1 | Kong Lab-Boston Children's Hospital | |
| Single guide RNA for Srd5a2 | Kong Lab-Boston Children's Hospital | |
| Srd5a1 Lentivirus | Origene | Cat No. MR203282 |
| Srd5a2 Lentivirus | Origene | Cat No. MR223814 |
| Sequence-Based Reagent | ||
| Primers for qRT-PCR | See Table 1 | |
| Software | ||
| ILLUSTRATOR 2021 | Adobe | N/A |
| PRISM 7 | GraphPad Software | N/A |
| Motor Monitor software | Hamilton-Kinder | N/A |
| Python | Python Software Foundation | N/A |
| Mobility App | Maguire Laboratory | (https://github.com/researchgrant/mobility-mapper.git) |
| Zhang Lab Guide Design Resource | Zhang Lab MIT | http://crispr.mit.edu/ |
| CHOPCHOP | http://chopcho.cbu.uib.no/ | Labun, K., Montague, T. G., Krause, M., Torres Cleuren, Y. N., Tjeldnes, H., & Valen, E. CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing. Nucleic Acids Research (2019). |
| NIS-Elements | Nikon Instruments | RRID:SCR_014329 |
| LAS X Life Science Microscope Software | Leica Microsystems | |
| Image J | NIH | Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, https://imagej.nih.gov/ij/, 1997–2018. |