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. 1999 Oct;181(19):6042–6052. doi: 10.1128/jb.181.19.6042-6052.1999

FIG. 5.

FIG. 5

(A) Northern analysis of rsmA transcripts produced by E. carotovora subsp. carotovora Ecc71 (lane 1) and its RsmC− derivative, AC5053 (lane 2). Total RNAs were extracted from bacteria grown in minimal salts medium plus sucrose at 28°C to an A600 of 2.0. Each lane contained 10 μg of total RNA. (B) Western blot analysis of RsmA produced by E. carotovora subsp. carotovora Ecc71 (RsmC+; lane 1) and AC5053 (RsmC−; lane 2). Each lane contained 20 μg of total bacterial protein. (C) Levels of rsmA transcripts in E. carotovora subsp. carotovora Ecc71 (RsmC+) carrying the cloning vector pCL1920 (lane 1) or the rsmC+ plasmid pAKC975 (lane 2) and AC5053 (RsmC−) carrying the cloning vector pCL1920 (lane 3) or the rsmC+ plasmid pAKC975 (lane 4). Total RNAs were extracted from bacteria grown in minimal salts medium plus sucrose and spectinomycin at 28°C to an A600 of 2.0 and subjected to Northern analysis. Each lane contained 10 μg of total RNA.