FIG. 6.

(A) β-Galactosidase assays of E. carotovora subsp. carotovora AC5047 or its RsmC⁻ mutant AC5050 carrying the cloning vector pNM481Sp^r, pAKC887 (rsmA_Ecc-lacZ fusion), pAKC888 (rsmA_Ea-lacZ fusion), pAKC889 (rsmA_Ehg-lacZ fusion), and pAKC890 (csrA-lacZ fusion). Bacteria were grown at 28°C in minimal salts medium plus sucrose and spectinomycin to an A₆₀₀ of 2.0 and assayed for β-galactosidase activity. (B) β-Galactosidase assays of E. carotovora subsp. carotovora AC5047 or its RsmC⁻ mutant AC5050 carrying the cloning vector pMP220 or the rsmB-lacZ plasmid pAKC1002. Bacteria were grown at 28°C in minimal salts medium plus sucrose and tetracycline to an A₆₀₀ of 2.0 and assayed for β-galactosidase activity.