TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| E. carotovora subsp. carotovora | ||
| Ecc71 | Wild type | 45 |
| AC5047 | Nalr derivative of AC5006 | 4 |
| AC5050 | RsmC− derivative of AC5047 by mini-Tn5-Km mutagenesis | This work |
| AC5051 | Ohl− derivative of AC5050 by marker exchange with pAKC855 | This work |
| AC5053 | RsmC− derivative of Ecc71 by marker exchange with pAKC970 | This work |
| AC5054 | RsmC− derivative of AC5071 by marker exchange with pAKC980 | This work |
| AC5070 | RsmA− derivative of AC5047 | 4 |
| AC5071 | RsmA− derivative of Ecc71 | 24 |
| AC5091 | Ohl− derivative of AC5047 | 4 |
| SCRI193 | Wild type | 37 |
| E. carotovora subsp. atroseptica Eca12 | Wild type | 45 |
| E. carotovora subsp. betavasculorum Ecb11129 | Wild type | J. E. Loper |
| E. herbicola pv. gypsophilae PD713 | Wild type | 17 |
| Escherichia coli | ||
| DH5α | φ80lacZΔM15Δ(lacZYA-argF) U169 hsdR17 recA1 endA1 thi-1 | Gibco BRL |
| JM109(DE3) | endA1 recA1 gyrA96 hsdR17 supE44 relA1 thi Δ(lac-pro) F′ (traD36 proAB+lacIqlacZΔM15) λcI857 ind1 Sam7 lacU5-T7 gene 1 | Promega Biotec |
| MC4100 | araD139 Δ(lacIPOZYA)U169 recA1 thi-1 Strr | 18 |
| Plasmids | ||
| pBluescript SK(+) | Apr | Stratagene |
| pCL1920 | Spr, Smr | 16 |
| p34S-Gm | Gmr; source of Gmr cassette | 8 |
| pCL1920Gmr | Gmr, Spr, Smr, Gmr cassette inserted at the BamHI site of pCL1920 | This work |
| pET-28a(+) | Kmr | Novagen |
| pHP45Ω | Source of Spr omega fragment | 33 |
| pLARF5 | Tcr | 15 |
| pMP220 | Tcr; promoter-probe vector | 40 |
| pNM481 | Apr, promoter-probe vector | 23 |
| pNM481Spr | Spr, Spr omega fragment inserted at the ScaI site of pNM481 | This work |
| pRK415 | Tcr | 15 |
| pRK2013 | Mob+, Tra+, Kmr | 10 |
| pAKC781 | Apr, peh-1+ | 18 |
| pAKC783 | Apr, pel-1+ | 18 |
| pAKC882 | Apr, pT7-rsmA | 26 |
| pAKC855 | Spr, Tcr, Ohl− | 4 |
| pAKC887 | Spr, rsmAEcc-lacZ, 279-bp (245 bases upstream of translational start site to 34 bases downstream of translational start site) PCR product from Ecc71 in pNM481Spr | This work |
| pAKC888 | Spr, rsmAEa-lacZ, 317-bp (283 bases upstream of translational start site to 34 bases downstream of translational start site) PCR product from E9 in pNM481Spr | This work |
| pAKC889 | Spr, rsmAEhg-lacZ, 300-bp (266 bases upstream of translational start site to 34 bases downstream of translational start site) PCR product from PD713 in pNM481Spr | This work |
| pAKC890 | Spr, csrA-lacZ, 287 bp (252 bases upstream of translational start site to 34 bases downstream of translational start site) PCR product from MC4100 in pNM481Spr | This work |
| pAKC924 | Apr, hrpNEcc in pBluescript SK(+) | 7 |
| pAKC970 | Kmr, Tcr; pLARF5 containing mini-Tn5-Km and flanking chromosomal DNA from AC5050 | This work |
| pAKC971 | Tcr; pLARF5 containing rsmC from genomic library of Ecc71 | This work |
| pAKC972 | Tcr; pLARF5 containing rsmC from genomic library of Ecc71 | This work |
| pAKC973 | Apr, 4.0-kb ClaI fragment of pAKC970 containing mini-Tn5-Km and flanking DNA in pBluescript SK (+) | This work |
| pAKC974 | Apr; RsmC+, 2.0-kb ClaI fragment of pAKC971 in pBluescript SK (+) | This work |
| pAKC975 | Spr, RsmC+, 2.0-kb ClaI fragment of pAKC971 in pCL1920 | This work |
| pAKC976 | Apr, 1.5-kb ClaI-HindIII fragment of pAKC973 (flanking DNA of mini-Tn5-Km) in pBluescript SK (+) | This work |
| pAKC977 | Apr, 0.5-kb ClaI-HindIII fragment of pAKC973 (flanking DNA of mini-Tn5-Km) in pBluescript SK (+) | This work |
| pAKC978 | Kmr, 371-bp EcoRV-HindIII fragment of pAKC974 in pET28a(+) | This work |
| pAKC979 | Tcr, RsmC+, 2.0-kb ClaI fragment of pAKC971 in pRK415 | This work |
| pAKC980 | Tcr, Spr; RsmC was inactivated in pAKC979 by inserting an omega fragment at the EcoRV site | This work |
| pAKC1002 | Tcr, rsmB-lacZ in pMP220 | 19 |
| pAKC1004 | Spr, plac-rsmB′ in pCL1920 | 19 |
| pAKC1004Gmr | Gmr, Spr, Gmr cassette inserted at the BamHI site of pAKC1004 | This work |
| pAKC1034 | Apr, 200-bp DNA fragment of celV from Ecc71 in pGEM-T Easy | 19 |