TABLE 2.
Levels of Pel, Peh, Prt, and Cel produced by E. carotovora subsp. carotovora strains
| Straina | Relevant phenotypeb | Sp act (U/ml/A600 unit; mean ± SD)c
|
|||
|---|---|---|---|---|---|
| Pel | Peh | Prt | Cel | ||
| Ecc71 | RsmA+ RsmC+ | 0.19 ± 0.03 | 66.2 ± 3.21 | 1.4 ± 0.28 | ND |
| AC5053 | RsmA+ RsmC− | 5.00 ± 0.19 | 441.8 ± 8.64 | 57.9 ± 2.32 | 1.32 ± 0.26 |
| AC5071 | RsmA− RsmC+ | 6.97 ± 0.23 | 391.8 ± 2.31 | 23.8 ± 2.74 | 0.90 ± 0.03 |
| AC5054 | RsmA− RsmC− | 15.77 ± 0.43 | 744.5 ± 18.69 | 77.8 ± 4.15 | 3.32 ± 0.09 |
| AC5047(pCL1920) | RsmA+ RsmC+ (none [vector]) | 0.17 ± 0.02 | 10.3 ± 0.26 | — | — |
| AC5047(pAKC975) | RsmA+ RsmC+ (RsmC+) | 0.06 ± 0.01 | ND | — | — |
| AC5050(pCL1920) | RsmA+ RsmC− (none [vector]) | 2.10 ± 0.11 | 341.0 ± 9.70 | — | — |
| AC5050(pAKC975) | RsmA+ RsmC− (RsmC+) | 0.11 ± 0.01 | 36.7 ± 1.70 | — | — |
a
Bacteria were grown at 28°C in minimal salts medium plus sucrose (0.5% [wt/vol]) for Pel, Peh, and Cel assays or in this medium plus celery extract for Prt assay. Strains carrying plasmids were grown in minimal salts medium plus sucrose and spectinomycin. Cultures were harvested at an A600 value of 2.5, and the culture supernatants were used for enzyme assays.
b
Relevant phenotypes conferred by the plasmids are indicated in parentheses.
c
ND, not detectable; —, not done.