TABLE 3.
Levels of Pel produced by E. carotovora subsp. carotovora strains carrying multiple copies of rsmB+ DNA
| Bacterial constructa | Relevant phenotypeb | Pel sp act (U/ml/A600 unit; mean ± SD) |
|---|---|---|
| Ecc71(pCL1920Gmr) | RsmA+ RsmB+ RsmC+ (none [vector]) | 0.2 ± 0.00 |
| Ecc71(pAKC1004Gmr) | RsmA+ RsmB+ RsmC+ (RsmB+) | 4.2 ± 0.08 |
| AC5053(pCL1920Gmr) | RsmA+ RsmB+ RsmC− (none [vector]) | 4.6 ± 0.04 |
| AC5053(pAKC1004Gmr) | RsmA+ RsmB+ RsmC− (RsmB+) | 10.6 ± 0.58 |
| AC5071(pCL1920Gmr) | RsmA− RsmB+ RsmC+ (none [vector]) | 7.8 ± 0.14 |
| AC5071(pAKC1004Gmr) | RsmA− RsmB+ RsmC+ (RsmB+) | 19.1 ± 0.08 |
| AC5054(pCL1920Gmr) | RsmA− RsmB+ RsmC− (none [vector]) | 22.9 ± 0.36 |
| AC5054(pAKC1004Gmr) | RsmA− RsmB+ RsmC− (RsmB+) | 21.3 ± 0.03 |
a
Bacteria were grown at 28°C in minimal salts medium plus sucrose (0.5% [wt/vol]) and gentamicin to an A600 value of 2.5, and culture supernatants were used for enzyme assays.
b
Relevant phenotypes conferred by the plasmids are given in parentheses.