C) Primer sets used in Quantitative PCR (qPCR)

Gene name	Accession number		Primer sequence (5’−3’)	Product size
36B4	NM_007475.5	F	GCAGGTGTTTGACAACGGCA	190bp
		R	CACAGACAATGCCAGGACGC
P2X7	NM_011027.4	F	GTTTGCTGTGGTCTAGCCTGG	163bp
		R	CCCACTTGACGGTGCCATAA
NLRP3	NM_001359638.1	F	TACCCAAGGCTGCTATCTGGAG	146bp
		R	AGCTTGCAACGGACACTCGT
Caspase-1	NM_009807.2	F	AGGACTGACTGGGACCCTCA	171bp
		R	ACTTGAGCTCCAACCCTCGG
IL-1β	NM_008361.4	F	TGCCACCTTTTGACAGTGATGA	158bp
		R	TGCCTGCCTGAAGCTCTTGT

F, Forward primer; R, Reverse primer